Identification of a Novel Sequence at the 3′ End of the GB Virus B Genome

AUTOR(ES)

Sbardellati, Andrea

FONTE

American Society for Microbiology

RESUMO

GB virus B (GBV-B) is a virus of the family Flaviviridae that infects small primates (Saguinus sp. [tamarins]) and shows similarities to hepatitis C virus (HCV) in genome organization, protein function, tissue tropism, and pathogenicity. This suggests the possibility of using tamarins infected by GBV-B or GBV-B/HCV chimeric viruses as a surrogate animal model of HCV infection. To achieve the construction of such chimeric viruses, it is essential to produce a complete and infectious GBV-B genomic RNA. We have identified a novel sequence at the 3′ end of the GBV-B genome and show that it can be arranged in a secondary structure resembling that of the 3′ end of the HCV genome, which is known to be essential for infectivity.

Documentos Relacionados

• Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA.
• The nucleotide sequence of an untranslated but conserved domain at the 3' end of the avian sarcoma virus genome.
• Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.
• Transcriptional mapping of the 3' end of the bovine syncytial virus genome.
• Structural characterization of the highly conserved 98-base sequence at the 3′ end of HCV RNA genome and the complementary sequence located at the 5′ end of the replicative viral strand