Identification of a vaccinia virus gene encoding a type I DNA topoisomerase.
AUTOR(ES)
Shuman, S
RESUMO
Vaccinia virus encapsidates a type I DNA topoisomerase (EC 5.99.1.2). The enzyme was purified from virus cores to apparent homogeneity, yielding a protein of Mr 32,000. The amino-terminal sequence of the isolated Mr 32,000 polypeptide was determined and used to map the putative structural gene for the vaccinia topoisomerase to the H7r open reading frame of the vaccinia genome. This gene encodes a 314-amino acid polypeptide containing a region homologous to a region of the type I topoisomerase from the yeast Saccharomyces cerevisiae.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=299319Documentos Relacionados
- Effects of ATP and inhibitory factors on the activity of vaccinia virus type I topoisomerase.
- Characterization of a Leishmania donovani gene encoding a protein that closely resembles a type IB topoisomerase.
- Identification of the vaccinia virus gene encoding nucleoside triphosphate phosphohydrolase I, a DNA-dependent ATPase.
- A protein kinase activity tightly associated with Drosophila type II DNA topoisomerase.
- Sequence dependent modulating effect of camptothecin on the DNA-cleaving activity of the calf thymus type I topoisomerase.
