Identification of Achromobacter species by cellular fatty acids and by production of keto acids.
AUTOR(ES)
Dees, S B
RESUMO
The cellular fatty acid composition and metabolic products of 12 reference strains of Achromobacter sp. and A. xylosoxidans were determined by gas-liquid chromatography (GLC). Results showed that the two Achromobacter groups are strikingly different and can be readily distinguished on the basis of cellular fatty acids and the short-chain acids produced by Achromobacter sp. The major cellular fatty acids of Achromobacter sp. were octadecenoic (18:1) and a 19-carbon cyclopropanoic (19:0 delta) acid, whereas hexadecanoic (16:0) and a 17-carbon cyclopropanoic (17:0 delta) acid were principal components of the lipids of A. xylosoxidans. Hydroxy acids were not found in strains of Achromobacter sp. but comprised approximately 20% of the cellular fatty acids of A. xylosoxidans. In addition, Achromobacter sp. produced relatively large amounts of 2-ketoisocaproic acid, which was detected in only trace amounts from strains of A. xylosoxidans. The data show that GLC tests provide additional criteria for differentiating groups which are very closely related when evaluated with conventional tests. The GLC tests can be readily adapted in the clinical laboratory because they are rapid, highly reproducible, relatively inexpensive, and simple to perform.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=275115Documentos Relacionados
- Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids.
- Fatty Acid Production from Amino Acids and α-Keto Acids by Brevibacterium linens BL2†
- Cellular fatty acids of Capnocytophaga species.
- Cellular fatty acids in Fusobacterium species as a tool for identification.
- Involvement of autolysin in cellular lysis of Bacillus subtilis induced by short- and medium-chain fatty acids.
