Identification of alternative transcripts of rat CD9 expressed by tumorigenic neural cell lines and in normal tissues
AUTOR(ES)
Wolfahrt, Sonja, Herman, Sandra, Scholz, Claus-Jürgen, Sauer, Georg, Deissler, Helmut
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
2013
RESUMO
CD9 is the best-studied member of the tetraspanin family of transmembrane proteins. It is involved in various fundamental cellular processes and its altered expression is a characteristic of malignant cells of different origins. Despite numerous investigations confirming its fundamental role, the heterogeneity of CD9 or other tetraspanin proteins was considered only to be caused by posttranslational modification, rather than alternative splicing. Here we describe the first identification of CD9 transcript variants expressed by cell lines derived from fetal rat brain cells. Variant mRNA-B lacks a potential translation initiation codon in the alternative exon 1 and seems to be characteristic of the tumorigenic BT cell lines. In contrast, variant mRNA-C can be translated from a functional initiation codon located in its extended exon 2, and substantial amounts of this form detected in various tissues suggest a contribution to CD9 functions. From the alternative sequence of variant C, a different membrane topology (5 transmembrane domains) and a deviating spectrum of functions can be expected.
Documentos Relacionados
- Identification of an alternative form of human lactoferrin mRNA that is expressed differentially in normal tissues and tumor-derived cell lines
- Direct Binding of the Ligand PSG17 to CD9 Requires a CD9 Site Essential for Sperm-Egg Fusion
- CD9 predicts ETV6-RUNX1 in childhood B-cell precursor acute lymphoblastic leukemia
- Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues.
- Detection of c-rel-related transcripts in mouse hematopoietic tissues, fractionated lymphocyte populations, and cell lines.