Identification of an enhancer-like element in the 5' flanking region of the rat apolipoprotein A-I gene.
AUTOR(ES)
Chao, Y S
RESUMO
In order to study the expression of the apolipoprotein (apo) A-I gene, we have isolated and characterized the structural gene encoding rat apo A-I. The 5' flanking sequence of the apo A-I gene was placed upstream of the coding sequence of the bacterial chloramphenicol acetyl transferase (CAT) gene, such that the expression of CAT activity in cultured cells is under the control of the promoter and regulatory sequences of the rat apo A-I gene. By transient transfection, nucleotide deletion and substitution methods, it was demonstrated that the nucleotide sequences between -464 and -148 upstream from the start of transcription of the rat apo A-I gene are required for the expression of this gene in Hep G2 cells and that these sequences function with an enhancer-like activity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=338351Documentos Relacionados
- Apolipoprotein A-I deficiency due to a codon 84 nonsense mutation of the apolipoprotein A-I gene.
- Multiple enhancer-like sequences in the HLA-B7 gene.
- An upstream enhancer and a negative element in the 5' flanking region of the human urokinase plasminogen activator gene.
- Isolation and characterization of the human apolipoprotein A-I gene.
- Identification of a distal regulatory element in the 5' flanking region of the bovine prolactin gene.