Identification of cis-acting sequences required for translational autoregulation of the ermC methylase.
AUTOR(ES)
Breidt, F
RESUMO
ermC methylase gene expression has been shown to be limited by translational autorepression, presumably due to methylase binding to ermC mRNA. It was found that this repression occurs in trans, yielding a 50% reduction in translation of an ermC-lacZ fusion mRNA. We investigated the ermC mRNA sequences required for translational repression in vivo. A series of deletions identified sequences in the 5' regulatory region that were required for translational repression. These included sequences of the 5' stem-loop structure that were not required for induction, as well as some that were required. The implications of these results for regulation are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=213340Documentos Relacionados
- Identification of a cis-acting element required for shunt-mediated translational initiation of the Sendai virus Y proteins
- cis-Acting Sequences Required for Simian Foamy Virus Type 1 Vectors
- Delineation of Cis-Acting Sequences Required for Expression of Drosophila Mojavensis Adh-1
- cis-acting sequences required for encapsidation of duck hepatitis B virus pregenomic RNA.
- Translational autoregulation of ermC 23S rRNA methyltransferase expression in Bacillus subtilis.
