Identification of Multivesicular Bodies as Prevacuolar Compartments in Nicotiana tabacum BY-2 CellsW⃞
AUTOR(ES)
Tse, Yu Chung
FONTE
American Society of Plant Biologists
RESUMO
Little is known about the dynamics and molecular components of plant prevacuolar compartments (PVCs). We have demonstrated recently that vacuolar sorting receptor (VSR) proteins are concentrated on PVCs. In this study, we generated transgenic Nicotiana tabacum (tobacco) BY-2 cell lines expressing two yellow fluorescent protein (YFP)-fusion reporters that mark PVC and Golgi organelles. Both transgenic cell lines exhibited typical punctate YFP signals corresponding to distinct PVC and Golgi organelles because the PVC reporter colocalized with VSR proteins, whereas the Golgi marker colocalized with mannosidase I in confocal immunofluorescence. Brefeldin A induced the YFP-labeled Golgi stacks but not the YFP-marked PVCs to form typical enlarged structures. By contrast, wortmannin caused YFP-labeled PVCs but not YFP-labeled Golgi stacks to vacuolate. VSR antibodies labeled multivesicular bodies (MVBs) on thin sections prepared from high-pressure frozen/freeze substituted samples, and the enlarged PVCs also were indentified as MVBs. MVBs were further purified from BY-2 cells and found to contain VSR proteins via immunogold negative staining. Similar to YFP-labeled Golgi stacks, YFP-labeled PVCs are mobile organelles in BY-2 cells. Thus, we have unequivocally identified MVBs as PVCs in N. tabacum BY-2 cells. Uptake studies with the styryl dye FM4-64 strongly indicate that PVCs also lie on the endocytic pathway of BY-2 cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=385280Documentos Relacionados
- Análise da resposta antioxidativa de células de Nicotiana tabacum cv BY-2 submetidas ao cádmio.
- Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant CellsW⃞
- The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm CellsW⃞
- Análise da resposta antioxidativa de células in vitro de fumo (Nicotiana tabacum cv BY-2) submetidas ao metal pesado níquel
- Estresse oxidativo e diferenças na sensibilidade de células de tabaco (Nicotiana tabacum L.) cv. BY-2 ao alumínio e à acidez