Identification of the promoter region of the ribosome-releasing factor cistron (frr).
AUTOR(ES)
Shimizu, I
RESUMO
Previous studies of the structure and expression of the ribosome-releasing factor (RRF) cistron (frr) have suggested that an efficient promoter region is located in the RRF cistron. We report here on the nucleotide sequence and in vivo function of the RRF promoter. The transcriptional start site was determined by primer extension to be 58 bp upstream of the translational initiation codon of frr. The location of the RRF promoter region was confirmed by means of (i) deletion analysis of the 5' proximal sequences of frr fused to the chloramphenicol acetyltransferase reporter gene, (ii) analysis of RRF produced in vivo from the deletion derivatives of frr cloned into pUC19, and (iii) gel retardation analysis with Escherichia coli RNA polymerase. The -35 and -10 regions were TTacCc and TATAcT, respectively. The strength of the RRF promoter was similar to that of the lac promoter, as determined by in vivo expression of chloramphenicol acetyltransferase activity. However, the RRF promoter was not affected by the intracellular cyclic AMP level despite the presence of a cyclic AMP receptor protein binding site downstream of the RRF promoter.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=208211Documentos Relacionados
- Localization of the ribosome-releasing factor gene in the Escherichia coli chromosome.
- Reinitiation of translation from the triplet next to the amber termination codon in the absence of ribosome-releasing factor.
- Ribosome recycling factor (ribosome releasing factor) is essential for bacterial growth.
- Functional identification of regulatory elements within the promoter region of platelet-derived growth factor 2.
- Identification of a cell-type-specific transcriptional repressor in the promoter region of the mouse hepatocyte growth factor gene.