Identification of the sigB Operon in Staphylococcus epidermidis: Construction and Characterization of a sigB Deletion Mutant

AUTOR(ES)

Kies, Stefanie

FONTE

American Society for Microbiology

RESUMO

The role of the alternative sigma factor ςB in Staphylococcus epidermidis was investigated by the construction, complementation, and characterization of a sigB deletion mutant. Electrophoretic analyses confirmed a profound influence of ςB on the expression of exoproteins and cytoplasmic proteins. Detailed investigation revealed reduced lipase and enhanced protease activity in the ςB mutant. Furthermore, no significant influence of ςB on heterologous biofilm formation or on the activity of the global regulator agr was detected.

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