Immune response to respiratory syncytial virus in young Brazilian children

AUTOR(ES)

Queiróz, D.A.O., Durigon, E.L., Botosso, V.F., Ejzemberg, B., Vieira, S.E., Mineo, J.R., Yamashita, C., Hein, N., Lopes, C.L., Cacharo, A.L., Stewien, K.E.

FONTE

Brazilian Journal of Medical and Biological Research

DATA DE PUBLICAÇÃO

2002-10

RESUMO

We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV.

Documentos Relacionados

• Antibody response to respiratory syncytial virus structural proteins in children with acute respiratory syncytial virus infection.
• Respiratory Syncytial Virus Infection: Immune Response, Immunopathogenesis, and Treatment
• Intravenous immunoglobulin treatment of respiratory syncytial virus infections in infants and young children.
• Comparison of rapid diagnostic techniques for respiratory syncytial and influenza A virus respiratory infections in young children.
• Induction of a neutralizing immune response to human respiratory syncytial virus with anti-idiotypic antibodies.