Immunization of chimpanzees with hepatitis B virus-derived polypeptides.
AUTOR(ES)
Dreesman, G R
RESUMO
Previous studies established that the purified polypeptides derived from the 22-nm particles associated with hepatitis B surface antigen (HBsAg) produce both humoral and cellular immunity against HBsAg in guinea pigs. Therefore, the two major polypeptides with molecular weights of 22,000 and 25,000 (P22 and P25, respectively) were isolated, adsorbed to an alum adjuvant, and used to immunize four nonimmune chimpanzees. A vigorous anti-HBs response was observed in all four animals after one inoculation of an alum-adsorbed polypeptide vaccine containing 40 micrograms of protein. After one to two booster inoculations, anti-HBs switched from being predominantly immunoglobulin M to the immunoglobin G class, indicating the establishment of immunological memory. Challenge of the vaccinated chimpanzees with 30,000 chimpanzee infectious doses of hepatitis B virus provided evidence for the efficacy of this vaccine. None of the four animals developed serological markers associated with an active hepatitis B infection, and no biochemical or histopathological changes of hepatitis were observed. A nonvaccinated control chimpanzee that was inoculated with the same hepatitis B virus material developed hepatitis B infection, confirming infectivity of the challenge inoculum.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=350587Documentos Relacionados
- Hepatitis delta virus: protein composition of delta antigen and its hepatitis B virus-derived envelope.
- Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA.
- Humoral and cellular immunity to hepatitis B virus-derived antigens: comparative activity of Freund complete adjuvant alum, and liposomes.
- Interaction of Human Immunodeficiency Virus-Derived Vectors with Wild-Type Virus in Transduced Cells
- Protection of chimpanzees from type B hepatitis by immunization with woodchuck hepatitis virus surface antigen.