Immunization of Mice with Components of Pasteurella multocida1
AUTOR(ES)
Srivastava, Kunwar K.
RESUMO
Log-phase cells of Pasteurella multocida strain P-1059 were used to prepare isolated culture filtrate, cell wall, and cytoplasmic components. Culture filtrate was further separated by column chromatography. A portion of cytoplasm and culture filtrate was conjugated to ferritin by means of metaxylylene diisocyanate. Cell walls induced more protection in mice than the conjugated or unconjugated cytoplasm or culture filtrate. The cell walls caused edema and erythema when given intradermally in rabbits, whereas cytoplasm and culture filtrate produced dermal necrosis. The first of four chromatographically separated fractions of culture filtrate was possibly more immunogenic in mice than cell walls. This fraction was less reactive intradermally in rabbits than cell walls but more reactive than the other fractions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=377089Documentos Relacionados
- Intranasal immunization of mice against Pasteurella multocida.
- Improved Hemagglutination Test for Identifying Type A Strains of Pasteurella multocida1
- Proliferative Responses of Rabbit Lymphocytes to Pasteurella multocida Decrease with Prolonged Immunization
- Pasteurella multocida infection in the domestic rabbit: immunization with a streptomycin-dependent mutant.
- Immune mechanism in Pasteurella multocida-infected mice.