Immunization of rats with heat-labile enterotoxin provides uniform protection against heterologous serotypes of enterotoxigenic Escherichia coli.
AUTOR(ES)
Klipstein, F A
RESUMO
Rats immunized with Escherichia coli heat-labile (LT) enterotoxin, either in the form of the holotoxin derived from a transformed K-12 strain or the polymyxin-release form obtained from human strains which produce LT toxin alone (LT+/ST- [ST is heat-stable toxin)] or together with ST toxin (LT+/ST+), were challenged with viable organisms of 10 different serotypes, 5 LT+/ST- and 5 LT+/ST+. The serum antitoxin response was monitored by enzyme-linked immunosorbent assay, and the degree of protection was determined by challenge in ligated ileal loops. Immunization with the holotoxin provided a strong antitoxin response and protection against all 10 challenge strains. Immunization with toxin from the LT+/ST+ strain provided equally strong protection against all strains, but immunization with toxin from the LT+/ST- strain yielded only a weak antitoxin response, moderate protection against challenge with LT+/ST- strains, and no protection against LT+/ST- strains, increasing by fivefold the immunization dosage of the LT+/ST- toxin failed to enhance protection. These observations (i) establish the fact that immunization with the LT holotoxin provides uniformly strong protection against heterologous serotypes and (ii) indicate that, for reasons which remain to be determined, the immunogenicity of the polymyxin-release LT from an LT+/ST+ strain differs from that of an LT+/ST- strain.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=351564Documentos Relacionados
- Protection against challenge with Escherichia coli heat-labile enterotoxin by immunization of rats with cholera toxin/toxoid.
- Protective Effect of Immunization with Heat-Labile Enterotoxin in Gnotobiotic Rats Monocontaminated with Enterotoxigenic Escherichia coli
- Peroral Immunization of Rats with Escherichia coli Heat-Labile Enterotoxin Delivered by Microspheres
- Respective contributions to protection of primary and booster immunization with Escherichia coli heat-labile enterotoxin in rats.
- Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.