Immunoglobulin heavy chain genes: demethylation accompanies class switching.
AUTOR(ES)
Rogers, J
RESUMO
The methylation of immunoglobulin heavy chain genes was examined before and after class switching, by using the Hpa II/Msp I restriction mapping technique. The mu, delta, and gamma 1 genes all are methylated in cells that do not express them but are demethylated when they are expressed. In particular, the delta gene remains methylated, and thus presumably untranscribed, in a cell line that probably represents an early stage of B-cell differentiation and produces only mu heavy chains. Because mu and delta RNAs are cotranscribed from a single complex transcription unit at a later stage of B-cell differentiation, this finding implies that the mu-plus-delta complex transcription unit is of variable length.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=349295Documentos Relacionados
- Organization of the sequences flanking immunoglobulin heavy chain genes and their role in class switching.
- Replacement of germ-line epsilon promoter by gene targeting alters control of immunoglobulin heavy chain class switching.
- Nucleotide sequence and properties of the murine gamma 3 immunoglobulin heavy chain gene switch region: implications for successive C gamma gene switching.
- Immunoglobulin heavy-chain switching may be directed by prior induction of transcripts from constant-region genes.
- Evolutionary approach to the question of immunoglobulin heavy chain switching: evidence from cloned human and mouse genes.