IMMUNOGLOBULIN SYNTHESIS AND SECRETION, V. INCORPORATION OF LEUCINE AND GLUCOSAMINE INTO IMMUNOGLOBULIN ON FREE AND BOUND POLYRIBOSOMES*
AUTOR(ES)
Sherr, Charles J.
RESUMO
Mouse myeloma cells pulse-labeled in vitro with 3H-leucine or 3H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for mouse immunoglobulin. The results indicate that immunoglobulin is synthesized preferentially by bound polyribosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=335804Documentos Relacionados
- IMMUNOGLOBULIN SYNTHESIS AND SECRETION, III. INCORPORATION OF GLUCOSAMINE INTO IMMUNOGLOBULIN ON POLYRIBOSOMES*
- Immunoglobulin Synthesis and Secretion, IV. Sites of Incorporation of Sugars as Determined by Subcellular Fractionation*
- Polyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population
- Synthesis of gamma G antibody and immunoglobulin on polyribosomes in a cell-free system.
- In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris: V. Incorporation of 14C-Leucine into a Protein Fraction Containing Ribulose 1,5-Diphosphate Carboxylase 1
