Immunoperoxidase method with human immune serum globulin for broad-spectrum detection of cultivable human enteric viruses: application to enumeration of cultivable viruses in environmental samples.
AUTOR(ES)
Payment, P
RESUMO
The detection and enumeration of most cultivable human enteric viruses from water is possible if samples are first inoculated onto a suitable cell line such as MA-104 or BGM. Virus growth is then detected by an indirect immunoperoxidase method with human immune serum globulin as the source of antibody to most enteric viruses. The number of positive cell cultures in the immunoperoxidase assay is used to calculate the virus titer (as a most probable number) in the sample assayed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=238745Documentos Relacionados
- A Broad-Spectrum Microbicide with Virucidal Activity against Sexually Transmitted Viruses
- Treatment with a Broad-Spectrum Cephalosporin versus Piperacillin-Tazobactam and the Risk for Isolation of Broad-Spectrum Cephalosporin-Resistant Enterobacter Species
- Broad-spectrum enzyme-linked immunosorbent assay for detection of Legionella soluble antigens.
- Broad-spectrum antimicrobial activity of human intestinal defensin 5.
- Peptide-mediated broad-spectrum plant resistance to tospoviruses
