ImobilizaÃÃo de tripsina em membranas produzidas por zoogloea sp

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Trypsin (E.C. 3.4.21.4) was covalently immobilized onto a membrane of a cellulosic exopolysaccharide produced by Zoogloea sp. in sugarcane molasses. Carbonyl groups were introduced into the matrix by sodium metaperiodate oxidation and the enzyme was immobilized either directly or through bovine serum albumin (BSA) as a spacer. The trypsinmembrane and trypsin-BSA-membrane retained, respectively, 37,2% and 9,16% of the specific activity of the native enzyme acting on benzoil-DL-Arginine-p-Nitroanilide (BAPNA). No activity decrease was observed in both preparations after seven reutilizations as well as they showed to be more thermal stable than the native enzyme. The trypsin-BSAmembrane presented the same initial activity (99%) after 54 days stored in 0.1 M Tris-HCl pH 8.0 buffer at 4oC but the trypsin-membrane lost 15% of activity. Furthermore, the trypsin- BSA-membrane lost 31% of activity after reuse at nine days interval during 54 days of storage at 4oC whereas the trypsin-membrane lost 69% of activity under the same conditions. These results demonstrated that this cellulosic matrix can be used for trypsin immobilization and the presence of BSA as an arm improved the derivative performance

ASSUNTO(S)

cellulosic exopolysaccharide tripsina zoogloea sp imobilizaÃÃo trypsin zoogloea sp bioquimica membrana polissacarÃdica immobilization

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