Impaired B cell development and function in mice with a targeted disruption of the homeobox gene Hex
AUTOR(ES)
Bogue, Clifford W.
FONTE
The National Academy of Sciences
RESUMO
Hex is a homeobox gene that is expressed in all stages of B cell development except plasma cells. We studied lymphocyte development in the absence of Hex by using the RAG1-deficient blastocyst complementation system because homozygous disruption of Hex is embryonic lethal. Hex−/−;RAG1−/− chimeric mice had severely reduced numbers of mature B cells, pre-B cells, and CD5+ B cells with a striking 15-fold increase in the percentage of B220−CD19+ cells in the bone marrow. Hex−/−;RAG1−/− chimeric mice failed to generate IgG antibodies to T cell-independent antigens, although their serum IgM levels and antibody responses to T cell-dependent antigens were intact. Therefore, Hex is necessary for B cell development and function and its absence results in a dramatic increase in B220−CD19+ cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=141034Documentos Relacionados
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