Improved growth of Campylobacter pylori in a biphasic system.
AUTOR(ES)
Shadowen, R D
RESUMO
The recovery of Campylobacter pylori from clinical specimens is difficult, even when done with an optimal medium, atmosphere, and temperature. The growth of this organism was investigated by comparing a biphasic system with broth culture. The effects of gyration, inoculum, and pH were studied. Brucella agar and broth supplemented with 2.5% fetal bovine serum were used. Growth in the biphasic system was an average of 2 log units (7 X 10(8) versus 5 X 10(6) CFU/ml) greater than that in the broth system (P less than 0.01), and this occurred 12 to 24 h sooner in the biphasic system. When gyration was added, an average of 1 log unit of growth improvement was seen in comparable systems. Improved growth was also seen with low inoculum levels, in which stationary-phase cells in the broth system reached 10(5) CFU/ml compared with 10(7) CFU/ml in the biphasic system. At the three pH ranges studied, growth was best at pH 8 to 9 (6 X 10(9) CFU/ml), averaging 2 log units greater growth than that at pH 6 to 7 and 4 log units greater growth than that at pH 4.5 to 5.5 (P less than 0.01). The improved recovery of the organism for low inoculum levels in a biphasic system may be important for long-term storage and clinical isolation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267665Documentos Relacionados
- Bulk growth of Neisseria gonorrhoeae type 1 in a biphasic system.
- Identification of Campylobacter pylori by using the rapID NH system.
- Biphasic culture system for rapid Campylobacter cultivation.
- Growth of Campylobacter pylori in liquid media.
- Purification of Junin virus by an aqueous biphasic polymer system.
