Improved immunobinding test using monoclonal antibodies for detection of Mycoplasma bovis in milk
AUTOR(ES)
Infante, F.
RESUMO
The aim of this study was to evaluate an improved immunobinding test (IBT) using monoclonal antibodies to identify Mycoplasma bovis in naturally infected milk. The IBT and the improved IBT were highly specific and had an immunologic sensitivity of 5 × 103 colony-forming units per milliliter. The results for the 2 methods agreed in the 130 milk samples tested. However, the IBT required 158 min, whereas the improved IBT required only 110 min. In addition, the improved IBT used smaller quantities of antibodies and conjugates.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=227017Documentos Relacionados
- Rapid Detection of Antigenic Diversity of Akabane Virus Isolates by Dot Immunobinding Assay Using Neutralizing Monoclonal Antibodies
- Detection of microsporidia by indirect immunofluorescence antibody test using polyclonal and monoclonal antibodies.
- Optimization of a rapid test by using fluorescein-conjugated monoclonal antibodies for detection of Chlamydia trachomatis in clinical specimens.
- Acridine orange staining for diagnosis of Mycoplasma bovis infection in cow milk.
- Improved Enzyme-Linked Immunosorbent Assay Using C-Terminal Truncated Recombinant Antigens of Babesia bovis Rhoptry-Associated Protein-1 for Detection of Specific Antibodies