Improved in vivo two-photon imaging after blood replacement by perfluorocarbon
AUTOR(ES)
Haiss, F
FONTE
Blackwell Science Inc
RESUMO
Two-photon microscopy is a powerful method in biomedical research that allows functional and anatomical imaging at a subcellular resolution in vivo. The technique is seriously hampered by absorption and scattering of light by blood, which prevents imaging through large vessels. Here, we demonstrate in the rat cerebral cortex that blood replacement by perfluorocarbon emulsion, a compound also used in human critical care medicine, yields superior image quality, while preserving neuronal integrity. Shadows of large superficial vessels disappear completely and cells can be imaged underneath them. For the first time, it is possible to image complete populations of neurons and astrocytes in the upper layers of neocortex in vivo.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2727028Documentos Relacionados
- In vivo two-photon calcium imaging of neuronal networks
- Two-photon imaging of capillary blood flow in olfactory bulb glomeruli
- Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence
- Quantitative Imaging of Molecular Order in Lipid Membranes Using Two-Photon Fluorescence Polarimetry
- Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy