Improved method for purification of enterotoxin from Clostridium perfringens type A.
AUTOR(ES)
Granum, P E
RESUMO
The purification procedure of Clostridium perfringens type A enterotoxin has been improved. The cell sonic extract was precipitated twice with ammonium sulfate, first 40% saturated to concentrate the enterotoxin and then 15% saturated. The two precipitations were followed by gel filtration on Sephadex G-100. The enterotoxin appeared to be homogeneous on 7% polyacrylamide gel electrophoresis after this three-step purification procedure, with a recovery of 56% and a 12.3-fold purification. The solubility properties at different pH values, temperatures, and ammonium sulfate concentrations are also given as basis for the purification procedure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=291492Documentos Relacionados
- Simplified Method for Purification of Clostridium perfringens Type A Enterotoxin
- Proteolysis of Clostridium perfringens type A enterotoxin during purification.
- Characterization of a parasporal inclusion body from sporulating, enterotoxin-positive Clostridium perfringens type A.
- Purification and Biochemical Properties of Clostridium perfringens Type A Enterotoxin
- Purification and characterization of an extracellular alpha-amylase from Clostridium perfringens type A.
