Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimens.
AUTOR(ES)
Kellogg, J A
RESUMO
Duplicate endocervical swabs were collected for detection of Chlamydia trachomatis by PCR (Roche Diagnostics). One swab was swirled in Specimen Transport Medium (Roche) for PCR testing and discarded. A saline aliquot from the other specimen, sent as a dry swab to the laboratory, was Papanicolaou stained to determine specimen adequacy, and the remainder was PCR tested. Significantly more (24%) PCR-positive results (118 versus 95; P < 0.001) were obtained with the dry specimens than with the swirled specimens when first tested. In addition, PCR-positive results were obtained with 107 (10.6%) of 1,007 microscopically adequate specimens but with only 3 (0.9%) of 341 inadequate specimens (P < 0.001).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=228572Documentos Relacionados
- Evaluation of a nonisotopic probe for detection of Chlamydia trachomatis in endocervical specimens.
- Comparison of IDEIA III and cell culture for the detection of Chlamydia trachomatis in endocervical specimens.
- Multiplex PCR for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Genitourinary specimens.
- Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction.
- Effect of endocervical specimen quality on detection of Chlamydia trachomatis and on the incidence of false-positive results with the Chlamydiazyme method.
