Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue.

AUTOR(ES)

Brown, S A

RESUMO

Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P less than 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 +/- 5.4%), followed by homogenization with NaOH digestion (85.8 +/- 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 +/- 3.3%). Simple homogenization recovered the least gentamicin (59.0 +/- 5.2%; P less than 0.05).

Documentos Relacionados

• New sodium hydroxide digestion method for measurement of renal tobramycin concentrations.
• Improved procedure for extracting aminoglycosides from renal cortical tissue.
• Method for improved extraction of DNA from Nocardia asteroides.
• Gentamicin concentrations in human subcutaneous tissue.
• Optimisation of DNA and RNA extraction from archival formalin-fixed tissue.