Improved Solid Medium for the Detection and Enumeration of Pectolytic Bacteria

AUTOR(ES)

Hankin, L.

RESUMO

An improved solid agar medium (MP medium) has been developed which allows detection of pectolytic activity in bacteria. Organisms tested exhibited a variety of regulatory controls governing pectate lyase synthesis. The medium contains mineral salts, pectin, and yeast extract. After growth of the organisms, the agar plate is flooded with a polysaccharide precipitant, and pectolytic activity is shown by clear zones around active colonies. High concentrations of phosphate are shown to be necessary for pectic enzyme formation on solid media. The medium has successfully been used to detect pectolytic organisms in soil, forest litter, and rotting vegetable samples.

Documentos Relacionados

• Improved Medium for Enumeration of Clostridium perfringens
• Improved medium for selective isolation and enumeration of Bifidobacterium.
• Versatile Medium for the Enumeration of Sulfate-Reducing Bacteria
• Improved medium for recovery and enumeration of the farmer's lung organism, Saccharomonospora viridis.
• Medium for the Selective Enumeration of Lactic Acid Bacteria from Foods