In organello footprint analysis of human mitochondrial DNA: human mitochondrial transcription factor A interactions at the origin of replication.
AUTOR(ES)
Ghivizzani, S C
RESUMO
Using in organello footprint analysis, we demonstrate that within human placental mitochondria there is a high level of protein-DNA binding at regularly phased intervals throughout a 500-bp region encompassing the D-loop DNA origins and two promoter regions. Comparison with in vitro DNase I protection studies indicates that this protein-DNA interaction is due to non-sequence-specific binding by human mitochondrial transcription factor A (h-mtTFA). Since h-mtTFA can bend and wrap DNA, like its yeast counterpart ABF2, a primary function of h-mtTFA appears to be specific packaging of the mitochondrial DNA control region in vivo. Intervals of protein binding coincide with the spacing of the RNA start sites and prominent D-loop DNA 5' ends, suggesting a role for phased h-mtTFA binding in defining transcription and H-strand DNA replication origins. Significant protein-DNA interaction was also observed within the human homolog of conserved sequence block 1, both in organello and in vitro, using purified h-mtTFA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=359313Documentos Relacionados
- Human mitochondrial DNA: analysis of 7S DNA from the origin of replication.
- In vivo protein-DNA interactions at human DNA replication origin.
- Co-operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of replication.
- Monkey B-lymphotropic papovavirus DNA: nucleotide sequence of the region around the origin of replication.
- Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.