In vitro aggregation of the gene-sized DNA molecules of the ciliate Stylonychia mytilus.
AUTOR(ES)
Lipps, H J
RESUMO
Macronuclear DNA of hypotrichous ciliates exists in the form of gene-sized DNA molecules. It can be resolved on agarose gels into a continuum of sizes upon which is imposed a set of characteristic DNA bands. Most or all of the DNA molecules carry identical terminal inverted repeat sequences. By incubating macronuclear DNA under increasingly stronger ionic conditions, high molecular weight DNA aggregates and ring-like DNA structures are formed. Experimental evidence is presented that this aggregation is not due to the presence of identical single-stranded DNA ends on each macronuclear DNA fragment, and an alternative model for DNA aggregation is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=349778Documentos Relacionados
- Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova.
- Macronuclear gene-sized molecules of hypotrichs.
- Chromatin structure in the macronucleus of the ciliate Stylonychia mytilus.
- Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.
- Gene-sized DNA molecules of the Oxytricha macronucleus have the same terminal sequence.