In vitro antibody secretion by peripheral blood mononuclear cells as an expression of the immune response to Brucella spp. in humans.
AUTOR(ES)
Vendrell, J P
RESUMO
We developed an assay to detect antibodies spontaneously secreted in vitro by peripheral blood mononuclear cells (PBMC) against Brucella spp. High levels of anti-Brucella immunoglobulin G (IgG) and/or IgM and/or IgA antibodies were detected in the cell supernatant solution of PBMC cultures for 12 patients suffering from acute or focalized brucellosis and for 5 patients recently vaccinated against brucellosis. This spontaneous in vitro antibody production disappeared 5 to 20 months after onset of clinical signs and 20 to 27 days after vaccination. The transient character of this anti-Brucella antibody production by PBMC is consistent with a temporary in vivo stimulation of the immune system by Brucella antigens. Detection of this secretion could improve the diagnosis of evolutive brucellosis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265472Documentos Relacionados
- Proliferative and interferon responses by peripheral blood mononuclear cells after bone marrow transplantation in humans.
- Peripheral blood lymphocyte response to acute infections in humans.
- IgA antibody-producing cells in peripheral blood after antigen ingestion: evidence for a common mucosal immune system in humans.
- Antibody-producing cells in peripheral blood and salivary glands after oral cholera vaccination of humans.
- Yops of Yersinia spp. pathogenic for humans.
