In Vitro Antifungal Activity and Cytotoxicity of a Novel Membrane-Active Peptide
AUTOR(ES)
Hong, Sung Yu
FONTE
American Society for Microbiology
RESUMO
In the present study, we investigated the antifungal activity and cytotoxicity of a novel membrane-active peptide, KKVVFKVKFKK (MP). MP inhibited the growth of various pathogenic fungi isolated from patients and of fluconazole-resistant fungi at concentrations of 2 to 32 μg/ml. MP had potent fungicidal activity; the minimal fungicidal concentrations of the peptide were no more than fourfold greater than the MICs. Time course experiments of MP-induced killing of Candida albicans ATCC 36232 showed that the rate of killing was rapid and depended on the concentration of MP. MP had a strong synergism with other antifungal drugs; the fractional inhibitory concentration index values of MP with amphotericin B and fluconazole for C. albicans were 0.16 and 0.02, respectively. The 50% inhibitory concentrations of MP for NIH 3T3 and Jurkat cells were approximately 100 times higher than the MIC for C. albicans ATCC 36232, indicating that MP had high selectivity between the fungal and mammalian cells. These results suggest that MP has great advantages in the development of antifungal agents.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=89347Documentos Relacionados
- Cyclohexane triones, novel membrane-active antibacterial agents.
- Mechanism of Action of EM 49, Membrane-Active Peptide Antibiotic
- In Vitro Activities of Membrane-Active Peptides against Gram-Positive and Gram-Negative Aerobic Bacteria
- Induction of petite yeast mutants by membrane-active agents.
- Lipid composition and sensitivity of Prototheca wickerhamii to membrane-active antimicrobial agents.
