In vitro bioactivity and cytotoxicity of chemically treated glass fibers
AUTOR(ES)
Andrade, Ângela Leão, Turchetti-Maia, Regina Maria de Marco, Lopes, Míriam Teresa Paz, Salas, Carlos Edmundo, Domingues, Rosana Zacarias
FONTE
Materials Research
DATA DE PUBLICAÇÃO
2004-12
RESUMO
Samples of a commercial glass fiber FM® (Fiber Max) were used to test the efficacy of a chemical sol-gel surface treatment to enhance their bioactivity. After treatment with tetraethoxysilane (TEOS), individual fiber samples were soaked into a simulated body fluid (SBF) solution, from which they were removed at intervals of 5 and 10 days. Micrographs obtained by scanning electron microscopy (SEM) analysis of samples chemically treated with TEOS revealed the formation of a hydroxyapatite (HA) coating layer after 5 days into SBF solution. Fourier transform infrared spectroscopic (FTIR) analyses confirmed that the coating layer has P-O vibration bands characteristic of HA. The in vitro cytotoxicity was evaluated using a direct contact test, minimum essential medium elution test (ISO 10993-5) and MTT assay. Fibers immersed in SBF and their extracts exhibited lower cytotoxicity than the controls not subjected to immersion, suggesting that SBF treatment improves the biocompatibility of the fiber.
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