In Vitro Enzymatic Synthesis of New Penicillins Containing Keto Acids as Side Chains

AUTOR(ES)

Ferrero, Miguel A.

RESUMO

Seven different penicillins containing α-ketobutyric, β-ketobutyric, γ-ketovaleric, α-ketohexanoic, δ-ketohexanoic, ε-ketoheptanoic, and α-ketooctanoic acids as side chains have been synthesized in vitro by incubating the enzymes phenylacetyl coenzyme A (CoA) ligase from Pseudomonas putida and acyl-CoA:6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum with CoA, ATP, Mg2+, dithiothreitol, 6-aminopenicillanic acid, and the corresponding side chain precursor.

Documentos Relacionados

• THE ENZYMATIC SYNTHESIS IN VITRO OF HYALURONIC ACID CHAINS*
• Synthesis and coupling reactions of alpha,alpha-dialkylated amino acids with nucleobase side chains.
• Evidence for In Vitro Binding of Pectin Side Chains to Cellulose1
• Enzymatic synthesis of polymers containing nicotinamide mononucleotide.
• Keto Acids as Growth-limiting Factors in Autotrophic Growth of Thiobacillus thiooxidans