In vitro formation of multimeric DNA structures mediated by purified simian virus 40 chromatin.
AUTOR(ES)
Waldeck, W
RESUMO
Simian virus 40 chromatin was incubated after purification by sucrose density-gradient centrifugation with various circular double-stranded DNA substrates. Monomeric rings were converted in the presence of Mg2+ to structures possessing a higher degree of complexity. Dimeric catenanes, as well as multimeric linear structures and concatemers, were generated, indicating that recombination events had occurred in vitro involving covalent linkage between different DNA molecules. Furthermore, apparently fused dimeric rings were observed. Their structures suggest that they may be recombination intermediates such as those described in a prokaryotic system [Potter, H. & Dressler, D. (1976) Proc. Natl. Acad. Sci. USA 73, 3000-3004]. Recombination did not take place between heterologous DNA substrates, as exclusively homologous multimeric DNA structures were observed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345892Documentos Relacionados
- Spliced and unspliced virus specific RNA sequences are associated with purified simian virus 40 chromatin.
- Specific association of simian virus 40 tumor antigen with simian virus 40 chromatin.
- Ultraviolet irradiation inhibits encapsidation of simian virus 40 chromatin.
- Folding of the DNA double helix in chromatin-like structures from simian virus 40.
- Primer-DNA formation during simian virus 40 DNA replication in vitro.