In Vitro Microassay for Biological Activity of Cyclophosphamide Metabolites

AUTOR(ES)

Laux, David

RESUMO

A rapid tissue culture assay for detecting the activated metabolites of cyclophosphamide in serum is described. Serum obtained shortly after drug administration was added to microtest wells containing a known number of previously planted target cells. The presence and amount of drug was directly correlated with the degree of inhibition of target cell replication. Either transformed or untransformed cells could be used as the target cells. No inhibition of cell growth was observed if cyclophosphamide was added directly to target cells without prior in vivo activation.

Documentos Relacionados

• Comparative activity of macrolides against Toxoplasma gondii demonstrating utility of an in vitro microassay.
• Microassay for Sindbis virus and interferon activity.
• IN VITRO BIOLOGICAL ACTIVITY OF CEPHALOTHIN
• Estrogenic activity in vivo and in vitro of some diethylstilbestrol metabolites and analogs
• In vitro activity of NifL, a signal transduction protein for biological nitrogen fixation.