In vitro synthesis of A-particle structual protein by membrane-bound polyribosomes.
AUTOR(ES)
Lueders, K K
RESUMO
On the basis of association with endoplasmic reticulum membranes, poyribosomes isolated from mouse myeloma MOPC-104E were separated into two classes, membrane bound and free. The membrane-bound and free polyribosomes were then compared for their capacity to incorporate [35S]methionine into A-particle proteins in vitro. As revealed by a radioimmunological assay method, labeling of A-particle protein occurred with the membrane-bound polyribosomes but not with the free polyribosomes. Peptide mapping of the immunoprecipitated, in vitro [35S]methionine-labeled product confirmed that A-particle protein had been synthesized in vitro.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=354808Documentos Relacionados
- In Vitro Synthesis of Proteins by Membrane-Bound Polyribosomes from Vesicular Stomatitis Virus-Infected HeLa Cells
- In vitro SYNTHESIS OF DIFFERENT CATEGORIES OF SPECIFIC PROTEIN BY MEMBRANE-BOUND AND FREE RIBOSOMES*
- Free and membrane-bound polyribosomes in BHK cells infected with Sindbis virus.
- Beta-amyloid precursor protein cleavage by a membrane-bound protease.
- Polyribosomes from Peas: VI. Auxin-stimulated Recruitment of Free Monosomes into Membrane-bound Polysomes
