In Vitro Synthesis of Adenovirus Core Proteins

AUTOR(ES)

Eron, Larry

RESUMO

mRNA extracted from polysomes of KB cells at late stages of productive infection with adenovirus type 2 was translated in a cell-free system derived from Krebs II ascites cells. Two of the polypeptides obtained in this reaction corresponded to the adenovirus core protein V and the precursor to core protein VII. The following criteria were used to establish identity between the in vitro products and the virion proteins: comigration during sodium dodecyl sulfatepolyacrylamide gel electrophoresis, cochromatography in sodium dodecyl sulfate-hydroxyapatite, specific immunoprecipitation of the precursor to core protein VII, and tryptic peptide analysis.

Documentos Relacionados

• Synthesis In Vitro of Type 5 Adenovirus Capsid Proteins 1
• In vivo and in vitro synthesis of adenovirus type 2 early proteins.
• Adenovirus DNA replication in vitro: synthesis of full-length DNA with purified proteins.
• Adenovirus core protein synthesis in the absence of viral DNA synthesis late in infection.
• Interactions among the three adenovirus core proteins.