In vitro synthesis of infectious retroviral RNA.

AUTOR(ES)

Flamant, F

RESUMO

A plasmid was constructed in which a T7 RNA polymerase promoter was placed upstream of a recombinant amphotropic retrovirus genome containing a selectable neomycin resistance gene. To test the infectivity of the RNA produced by T7 RNA polymerase in vitro, the RNA was microinjected into the nuclei of psi 2 packaging cells. Infectious particles conferring G418 resistance were released.

Documentos Relacionados

• In vitro synthesis of infectious poliovirus RNA.
• In vitro synthesis of T4 late messenger RNA.
• In vitro synthesis of late bacteriophage phi 29 RNA.
• In vitro synthesis of full-length influenza virus complementary RNA.
• Replication of viral RNA. 13. The early product of phage RNA synthesis in vitro.