In Vitro System for Production of Mouse Mammary Tumor Virus
AUTOR(ES)
Fine, D. L.
RESUMO
An in vitro system for production, purification, and concentration of mouse mammary tumor virus is described. Monolayer cultures of C3H mouse mammary tumor cells propagated at 34 C in roller bottles in the presence of dexamethasone, a glucocorticoid hormone, release B-type particles which possess ribonucleic acid and a ribonucleic acid-dependent deoxyribonucleic acid polymerase. One thousandfold concentration by ultracentrifugation with subsequent gradient fractionation yielded > 7 × 1010 particles per ml in the 1.16- to 1.18-g/ml region. Mouse mammary tumor virus produced in this system was free of detectable C-type virus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=186879Documentos Relacionados
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