In Vitro Uptake of 14C-Labeled Amino Acids by Preparations of Partially Purified Anaplasma marginale Bodies
AUTOR(ES)
Johns, R. W.
RESUMO
Studies on the incorporation of three 14C-labeled amino acids into preparations of partially purified Anaplasma marginale were conducted. Relatively high levels of uptake of radioactive isoleucine, glycine, and methionine were detected in both trichloroacetic acid-soluble and -insoluble fractions of these preparations, indicating that the parasite is capable of protein synthesis outside its erythrocytic environment. When erythrocytes (red blood cells) from infected calves were incubated with [14C]methionine and Anaplasma bodies were subsequently purified, it was found that some of the label detected was associated with the marginal bodies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=414858Documentos Relacionados
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