Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion.

AUTOR(ES)

Wassenaar, T M

RESUMO

The role of the Campylobacter jejuni flagella in adhesion to, and penetration into, eukaryotic cells was investigated. We used homologous recombination to inactivate the two flagellin genes flaA and flaB of C. jejuni, respectively. Mutants in which flaB but not flaA is inactivated remain motile. In contrast a defective flaA gene leads to immotile bacteria. Invasion studies showed that mutants without motile flagella have lost their potential to adhere to, and penetrate into, human intestinal cells in vitro. Invasive properties could be partially restored by centrifugation of the mutants onto the tissue culture cells, indicating that motility is a major, but not the only, factor involved in invasion.

Documentos Relacionados

• Differential flagellin expression in a flaA flaB+ mutant of Campylobacter jejuni.
• Sequence-Based Typing of flaB Is a More Stable Screening Tool Than Typing of flaA for Monitoring of Campylobacter Populations
• The Campylobacter sigma 54 flaB flagellin promoter is subject to environmental regulation.
• Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.
• The Decrease in FlaA Observed in a flaB Mutant of Borrelia burgdorferi Occurs Posttranscriptionally