Incorporation of [35S]Sulfate and [14C]Bicarbonate into Karyotype-specific Polysaccharides of Chondrus crispus1
AUTOR(ES)
Jackson, Susan G.
RESUMO
Gametophytic and sporophytic tissues of Chondrus crispus (Stackhouse) cultured in vitro were labeled with 35S and 14C. The major sulfated polysccharides isolated from the two karyotypes were characterized by KCI fractionation, immunoprecipitation, and infrared spectroscopy. Reproducibility of data has been demonstrated by an experiment using gametophytic T4 strain with five replicates per time point. The rate of sulfate uptake was similar in haploid and diploid plants from a given area cultured for a similar time. Cultures from different sources cultured for different times showed different uptake and incorporation levels. Although sulfate uptake did not appear to be karyotype-related, the pattern of incorporation of 35S and 14C into polysaccharides was ploidy-specific.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=543142Documentos Relacionados
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