Incorporation of Adeno-Associated Virus in a Calcium Phosphate Coprecipitate Improves Gene Transfer to Airway Epithelia In Vitro and In Vivo

AUTOR(ES)

Walters, Robert W.

FONTE

American Society for Microbiology

RESUMO

Adeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia because of a lack of receptors at the apical surface. We hypothesized that incorporation of AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly, coprecipitation of AAV type 2 improved gene transfer to differentiated human airway epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as a CaPi coprecipitate may significantly enhance its utility for gene transfer to the airway epithelia in vivo.

Documentos Relacionados

• Endosomal processing limits gene transfer to polarized airway epithelia by adeno-associated virus
• A shortened adeno-associated virus expression cassette for CFTR gene transfer to cystic fibrosis airway epithelia
• Incorporation of adenovirus in calcium phosphate precipitates enhances gene transfer to airway epithelia in vitro and in vivo.
• Adeno-Associated Virus Type 5 (AAV5) but Not AAV2 Binds to the Apical Surfaces of Airway Epithelia and Facilitates Gene Transfer
• Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer