Incorporation of Labeled Precursors into the RNA and Proteins of Lactic Dehydrogenase Virus
AUTOR(ES)
Niwa, Akira
RESUMO
Lactic dehydrogenase virus was grown in primary mouse embryo cells and labeled with 3H-uridine and 3H-amino acids. Concentrated and purified virus was banded by isopycnic centrifugation in sucrose gradients, and infectivity and radioactivity were found to correspond at a density of 1.17 g/cm3. The extracted viral RNA was resolved by electrophoresis in polyacrylamide-agarose mixed gels, and the mol wt was estimated to be 6.0 × 106.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356636Documentos Relacionados
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