Increased Activity of Chromatin-bound Ribonucleic Acid Polymerase from Soybean Hypocotyl with Spermidine and High Ionic Strength 1
AUTOR(ES)
Guilfoyle, T. J.
RESUMO
Optimal activity of chromatin-bound RNA polymerase from soybeans is obtained with 1 mm Mn2−, but only when high ionic strength or polyamines are included in the medium. Such inclusion does not increase the Mg2+ activation of the polymerase, but it does lower the concentration needed for optimum activity from 10 mm to 1 mm. Mg2− activation is inhibited by added Mn2+, and the inhibition is relieved by high ionic strength or spermidine. The RNA polymerase with either cation is almost entirely polymerase I at low and high ionic strength as evidenced by insensitivity to α-amanitin. Treatment of soybean seedlings with 2,4-dichlorophenoxyacetic acid does not change these characteristics; although the activity rises 3- to 4-fold.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=366396Documentos Relacionados
- Greater Length of Ribonucleic Acid Synthesized by Chromatin-bound Polymerase from Auxin-treated Soybean Hypocotyls 1
- Effect of aflatoxin B1 on chromatin-bound ribonucleic acid polymerase and nucleic acid and protein synthesis in germinating maize seeds.
- Gibberellic Acid Activates Chromatin-bound DNA-dependent RNA Polymerase in Wounded Potato Tuber Tissue 1
- Isolation of Nucleoli and Localization of Ribonucleic Acid Polymerase I from Soybean Hypocotyl 1
- Increased Ribonucleic Acid Polymerase Activity Associated with Chromatin from Internodes of Dwarf Pea Plants Treated with Gibberellic Acid 1
