Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside.
AUTOR(ES)
Norgard, M V
RESUMO
When pBR322 plasmid-harboring Escherichia coli strains RR1 or chi1776 were grown in the presence of 1 mg of uridine or cytidine per ml and later treated with chloramphenicol, as much as three times more plasmid deoxyribonucleic acid was recovered than would normally be obtained by routine plasmid amplification procedures.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=218271Documentos Relacionados
- Conservative integration of bacteriophage Mu DNA into pBR322 plasmid.
- Recombination between bacteriophage lambda and plasmid pBR322 in Escherichia coli.
- Response to temperature shifts of expression of the amp gene on pBR322 in Escherichia coli K-12.
- Tetracycline resistance element of pBR322 mediates potassium transport.
- Cooperativity in nucleosomes assembly on supercoiled pBR322 DNA.