Increased binding of ethidium bromide to polynucleotide duplexes containing mismatched bases.

AUTOR(ES)

Helfgott, D C

RESUMO

The binding of the intercalating dye ethidium bromide to a series of synthetic polynucleotide duplexes containing varying concentrations of mismatched bases has been measured by fluorescence titration. The dye binds more strongly to duplexes with mismatches, the estimated increase in affinity being twenty-fold for the series of molecules poly (I).poly (C,Ax) with x denoting the mole fraction of mismatched A residues in the C strand. The results are consistent with one requirement of the Streisinger model for frameshift mutagenesis, namely that frameshifting agents can function by stablizing mismatched transient intermediates in DNA.

Documentos Relacionados

• The stability of oligodeoxyribonucleotide duplexes containing degenerate bases.
• The binding of polyamines and of ethidium bromide to tRNA.
• Enzymatic synthesis of polyuridylic acid containing modified bases.
• Circular dichroism studies of ethidium bromide binding to the isolated nucleolus.
• Ethidium bromide binding to core particle: comparison with native chromatin.