Increased Fragility of Escherichia coli After Infection with Bacteriophage M13
AUTOR(ES)
Roy, A.
RESUMO
Male strains of Escherichia coli infected with filamentous phage M13 released the progeny phage particles from intact cells. At the same time, the cells continued to grow and multiply at a slightly lower rate than the uninfected cells. Concomitant with the phage release, lipopolysaccharide from the cell wall of the infected cells was also released. The buoyant density of E. coli HfrC in diaginol, 1.25 g/cc, did not change as a result of infection. Detergents like sodium dodecyl sulfate and Sarkosyl specifically lysed the infected cells. The infected cells showed enhanced fragility as indicated by inactivation by various stresses, namely heat, osmotic shock, and freezing and thawing. It is concluded that the infection with M13 causes certain alterations in the surface structure of E. coli, thus making the cells more fragile.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=376127Documentos Relacionados
- Replication of bacteriophage M13 IX. Requirement of the Escherichia coli dnaG function for M13 duplex DNA replication.
- THE PROTEINS OF BACTERIOPHAGE M13
- Replication of Bacteriophage M13: Specificity of the Escherichia coli dnaB Function for Replication of Double-Stranded M13 DNA
- Loss of R Factors Promoted by Bacteriophage M13 and the M13 Carrier State
- Replication of bacteriophage M13. XIV. Differential inhibition of the replication of M13 and M13 miniphage in a mutant of Escherichia coli defective in the 5' leads to 3' exonuclease associated with DNA polymerase I.