Independence of buoyant cell density and growth rate in Escherichia coli.
AUTOR(ES)
Kubitschek, H E
RESUMO
The relationship between growth rate and buoyant density was determined for cells from exponential-phase cultures of Escherichia coli B/r NC32 by equilibrium centrifugation in Percoll gradients at growth rates ranging from 0.15 to 2.3 doublings per h. The mean buoyant density did not change significantly with growth rate in any of three sets of experiments in which different gradient conditions were used. In addition, when cultures were allowed to enter the stationary phase of growth, mean cell volumes and buoyant densities usually remained unchanged for extended periods. These and earlier results support the existence of a highly regulated, discrete state of buoyant density during steady-state growth of E. coli and other cells that divide by equatorial fission.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=215411Documentos Relacionados
- Evidence for osmoregulation of cell growth and buoyant density in Escherichia coli.
- Buoyant density constancy during the cell cycle of Escherichia coli.
- Buoyant density, growth rate, and the cell cycle in Streptococcus faecium.
- Growth and buoyant density of Escherichia coli at very low osmolarities.
- Buoyant density studies of several mecillinam-resistant and division mutants of Escherichia coli.