Indirect Hemagglutination with Mycoplasma Antigens: Effects of pH on Antigen Sensitization of Tanned Fresh and Formalinized Sheep Erythrocytes
AUTOR(ES)
Krogsgaard-Jensen, Anders
RESUMO
An investigation of the influence of different factors affecting the sensitivity of the indirect hemagglutination test has been performed with antigens of four mycoplasmas isolated from sheep or goat. Tanned erythrocytes of sheep, fresh and formalinized, were sensitized with the above antigens. It was demonstrated that, with formalinized erythrocytes, the sensitivity was increased by 50 to 100 times when the sensitization was done at a low pH level. The pH level was unimportant for sensitizing fresh erythrocytes. The greatest sensitivity of the indirect hemagglutination test was obtained with fresh rather than formalinized erythrocytes. Three different types of antigens were used, and the most suitable antigen was found to be the supernatant fluid from an ultrasonically treated centrifuged Mycoplasma suspension.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=376412Documentos Relacionados
- Use of Formalinized Mycoplasma gallisepticum Antigens and Chicken Erythrocytes in Hemagglutination and Hemagglutination-Inhibition Studies
- Use of Formalinized Sheep Erythrocytes in the Rubella Hemagglutination-Inhibition Test
- PRESERVATION OF ANTIGEN-COATED SHEEP ERYTHROCYTES BY FREEZING FOR USE IN INDIRECT HEMAGGLUTINATION PROCEDURE
- Indirect hemagglutination test that uses glutaraldehyde-fixed sheep erythrocytes sensitized with extract antigens for detection of Pasteurella antibody.
- Use of a New Buffer System with Formalinized Sheep Erythrocytes in the Rubella Hemagglutination-Inhibition Test
