Individual and population variation in invertebrates revealed by Inter-simple Sequence Repeats (ISSRs)

AUTOR(ES)

Abbot, Patrick

FONTE

University of Arizona Library

RESUMO

PCR-based molecular markers are well suited for questions requiring large scale surveys of plant and animal populations. Inter-simple Sequence Repeats or ISSRs are analyzed by a recently developed technique based on the amplification of the regions between inverse-oriented microsatellite loci with oligonucleotides anchored in microsatellites themselves. ISSRs have shown much promise for the study of the population biology of plants, but have not yet been explored for similar studies of animals. The value of ISSRs is demonstrated for the study of animal species with low levels of within-population variation. Sets of primers are identified which reveal variation in two aphid species, Acyrthosiphon pisum and Pemphigus obesinymphae, in the yellow fever mosquito Aedes aegypti, and in a rotifer in the genus Philodina.

Documentos Relacionados

• Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats
• Nicho trofico de Gracilinanus microtarsus (Didelphimorphia: Didelphidae) : variação intra-populacional e inter-individual
• Intra- and inter-individual chromosome variation in Hoplerythrinus unitaeniatus (Pisces, Erythrinidae): a population from the Brazilian São Francisco river basin
• Sequence characterization of hypervariable regions in the soybean genome: leucine-rich repeats and simple sequence repeats
• Genetic variation in clonal vertebrates detected by simple-sequence DNA fingerprinting.