Induced K+ Efflux from Cultured Rose Cells 1: Effects of Protein-Synthesis Inhibitors
AUTOR(ES)
Murphy, Terence M.
RESUMO
Inhibitors of translation, cycloheximide, emetine, and puromycin, and inhibitors of transcription, actinomycin D and cordycepin, stimulate a net efflux of K+ from cultured cells of Rosa damascena. In the case of cycloheximide and emetine, this efflux bears many similarities to the efflux induced by ultraviolet radiation, including a lag period of 0.25 to 2.5 hours and a limited total loss of K+. The efflux is transient, and continued incubation of cells with cycloheximide and emetine allows the cells to recover the K+; after this, the cells no longer release K+ in response to UV or to cycloheximide treatment. This suggests that the efflux process depends on continued protein synthesis. Other treatments such as cerulenin, low temperature (0°C), and high temperature (40°C) also inhibit the UV- and cycloheximide-induced K+ efflux, suggesting that the induction of efflux may involve the synthesis of new plasma membrane.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1054579Documentos Relacionados
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